GATA1 antibody (pSer142)

Catalog No. ABIN7220978

This Rabbit Polyclonal antibody specifically detects GATA1 in WB, ELISA, IHC, IP and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GATA1 antibody (pSer142) (ABIN7220978)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-GATA1 Antibody

Binding Specificity: pSer142

Purpose: Rabbit Anti-GATA-1 (phospho Ser142) Polyclonal Antibody

Specificity: Phospho-GATA-1 (S142) Polyclonal Antibody detects endogenous levels of GATA-1 protein only when phosphorylated at S142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human GATA-1 Phospho-Ser142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA-1

Background: GATA1, ERYF1, GF1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding proteinGATA1 encodes a protein which belongs to the GATA family of transcription factors. The GATA binding protein 1 plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Molecular Weight: 40kD

Gene ID: 2623

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin