GLI3 antibody (AA 30-110)

Catalog No. ABIN7221148

This Rabbit Polyclonal antibody specifically detects GLI3 in IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GLI3 antibody (AA 30-110) (ABIN7221148)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-GLI3 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-GLI-3 Polyclonal Antibody

Specificity: GLI-3 Polyclonal Antibody detects endogenous levels of GLI-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human GLI-3 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI-3

Background: GLI3, Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3 full length protein, GLI3FLGLI3 encodes GLI family zinc finger 3 which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling