Glycogen Synthase 1 antibody (pSer645)

Catalog No. ABIN7221420

The Rabbit Polyclonal anti-Glycogen Synthase 1 antibody is suitable to detect Glycogen Synthase 1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Glycogen Synthase 1 antibody (pSer645) (ABIN7221420)

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycogen Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Glycogen Synthase 1 Antibody

Binding Specificity: pSer645

Purpose: Rabbit Anti-Glycogen Synthase 1 (phospho Ser645) Polyclonal Antibody

Specificity: Phospho-Glycogen Synthase 1 (S645) Polyclonal Antibody detects endogenous levels of Glycogen Synthase 1 protein only when phosphorylated at S645.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Glycogen Synthase 1 Phospho-Ser645

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Alternative Name: Glycogen Synthase 1

Background: GYS1, GYS, Glycogen [starch] synthase, muscleThe glycogen synthase 1 encoded by GYS1 catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in GYS1 are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for GYS1.

Molecular Weight: 83kD

Gene ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signaling, AMPK Signaling, Cellular Glucan Metabolic Process