GRAP antibody (AA 10-90)

Catalog No. ABIN7219138

This Rabbit Polyclonal antibody specifically detects GRAP in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GRAP antibody (AA 10-90) (ABIN7219138)

Target: GRAP (GRB2-Related Adaptor Protein (GRAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GRAP Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Grap Polyclonal Antibody

Specificity: Grap Polyclonal Antibody detects endogenous levels of Grap protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Grap at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GRAP

Target: GRAP (GRB2-Related Adaptor Protein (GRAP))

Alternative Name: Grap

Background: GRAP, GRB2-related adapter proteinGRAP encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

Molecular Weight: 28kD

Gene ID: 10750

UniProt: Q13588