HSF1 antibody (pSer121)

Catalog No. ABIN7221574

The Rabbit Polyclonal anti-HSF1 antibody is suitable to detect HSF1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for HSF1 antibody (pSer121) (ABIN7221574)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HSF1 Antibody

Binding Specificity: pSer121

Purpose: Rabbit Anti-HSF1 (phospho Ser121) Polyclonal Antibody

Specificity: Phospho-HSF1 (S121) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S121.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HSF1 Phospho-Ser121

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: HSF1, HSTF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1The product of HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.

Molecular Weight: 50kD

Gene ID: 3297

UniProt: Q00613