HCK antibody (pTyr521)

Catalog No. ABIN7221456

The Rabbit Polyclonal anti-HCK antibody (ABIN7221456) specifically detects HCK in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HCK antibody (pTyr521) (ABIN7221456)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HCK Antibody

Binding Specificity: pTyr521

Purpose: Rabbit Anti-Hck (phospho Tyr521) Polyclonal Antibody

Specificity: Phospho-Hck (Y521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Hck Phospho-Tyr521

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: Hck

Background: HCK, Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61HckThe HCK proto-oncogene, Src family tyrosine kinase encoded by HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.

Molecular Weight: 60kD

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling