HIRA antibody

Catalog No. ABIN7224842

The Rabbit Polyclonal anti-HIRA antibody (ABIN7224842) specifically detects HIRA in ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for HIRA antibody (ABIN7224842)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HIRA Antibody

Purpose: Rabbit Anti-HIRA Polyclonal Antibody

Specificity: HIRA Polyclonal Antibody detects endogenous levels of HIRA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HIRA around the non-phosphorylation site of T555

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA

Background: HIRA, DGCR1, HIR, TUPLE1, Protein HIRA, TUP1-like enhancer of split protein 1HIRA (histone cell cycle regulator) encodes a histone chaperone that preferentially places the variant histone H3. in nucleosomes. Orthologs of HIRA in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Gene ID: 7290

UniProt: P54198

Pathways: Chromatin Binding