Histone H2A antibody (pThr121)

Catalog No. ABIN7225254

The Rabbit Polyclonal anti-Histone H2A antibody (ABIN7225254) specifically detects Histone H2A in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Histone H2A antibody (pThr121) (ABIN7225254)

Target: Histone H2A

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Histone H2A Antibody

Binding Specificity: pThr121

Purpose: Rabbit Anti-Histone H2A (phospho Thr121) Polyclonal Antibody

Specificity: Phospho-Histone H2A (T121) Polyclonal Antibody detects endogenous levels of Histone H2A protein only when phosphorylated at T121.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Histone H2A Phospho-Thr121

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Histone H2A

Target: Histone H2A

Background: HIST1H2AG, H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN, Histone H2A type 1, H2A.1, Histone H2A/p, HIST1H2AB, H2AFM, HIST1H2AE, H2AFA, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HISHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H2AI (histone cluster 1 H2A family member i) is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from HIST1H2AI lack polyA tails but instead contain a palindromic termination element. HIST1H2AI is found in the small histone gene cluster on chromosome 6p22-p21.3.

Gene ID: 8329, 8332, 8336

UniProt: P0C0S8, P04908, P20671, Q96KK5, Q6FI13, Q7L7L0