Histone H2B antibody (AA 10-90)

Catalog No. ABIN7215258

This anti-Histone H2B antibody is a Rabbit Polyclonal antibody detecting Histone H2B in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Monkey.

Quick Overview for Histone H2B antibody (AA 10-90) (ABIN7215258)

Target: Histone H2B

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 10-90

Purpose: Histone H2B Polyclonal Antibody

Specificity: Histone H2B Polyclonal Antibody detects endogenous levels of Histone H2B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Histone H2B around the non-acetylation site of K12 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H2B

Target: Histone H2B

Background: Rabbit Anti-Histone H2B Polyclonal Antibody,H2BFS, Histone H2B type F-S, Histone H2B.s, H2B/s,H2BFS is a Pseudogene. Histone H2B type 1-H is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH.,Histone H2B type F-S

Molecular Weight: observerd band 14kDa

Gene ID: 54145

UniProt: P57053