Histone H2B antibody (acLys21)

Catalog No. ABIN7228910

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H2B antibody (acLys21) (ABIN7228910)

Target: Histone H2B

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Histone H2B Antibody

Binding Specificity: acLys21

Purpose: Histone H2B (Acetyl Lys21) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Histone H2B when Acetyl occurs at Lys21

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human Histone H2B (Acetyl Lys21) protein at AA range: 21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Histone H2B

Target: Histone H2B

Background: Rabbit Anti-Histone H2B (Acetyl Lys21) Polyclonal Antibody,Histone H2B type 1-A, HIST1H2BA, Histone H2B, testis, TSH2B.1, hTSH2B1, Testis-specific histone H2B,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BA is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from HIST1H2BA contain a palindromic termination element. HIST1H2BA (Histone Cluster 1 H2B Family Member A) is a Protein Coding gene. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and DNA Double Strand Break Response.,Histone H2B

Molecular Weight: observerd band 15kDa

Gene ID: 255626