Hexokinase 1 antibody (AA 1-80)

Catalog No. ABIN7215370

The Rabbit Polyclonal anti-Hexokinase 1 antibody is suitable to detect Hexokinase 1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Hexokinase 1 antibody (AA 1-80) (ABIN7215370)

Target: Hexokinase 1 (HK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-HXK I Polyclonal Antibody

Specificity: HXK I Polyclonal Antibody detects endogenous levels of HXK I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human HXK I at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HXK I

Background: HK1, Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK IHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. HK1 encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in HK1 have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Molecular Weight: 109kD

Gene ID: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect