HIF3A antibody (Internal Region)

Catalog No. ABIN7224250

This Rabbit Polyclonal antibody specifically detects HIF3A in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HIF3A antibody (Internal Region) (ABIN7224250)

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HIF3A Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-HIF-3α Polyclonal Antibody

Specificity: HIF-3α Polyclonal Antibody detects endogenous levels of HIF-3α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human HIF-3alpha

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF-3alpha

Background: Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, PAS domain-cThe protein encoded by HIF3A (hypoxia inducible factor 3 alpha subunit) is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for HIF3A.

Molecular Weight: 72kD

Gene ID: 64344

UniProt: Q66K72

Pathways: Warburg Effect