IRS1 antibody (pTyr896)

Catalog No. ABIN7221864

The Rabbit Polyclonal anti-IRS1 antibody (ABIN7221864) specifically detects IRS1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IRS1 antibody (pTyr896) (ABIN7221864)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IRS1 Antibody

Binding Specificity: pTyr896

Purpose: Rabbit Anti-IRS-1 (phospho Tyr896) Polyclonal Antibody

Specificity: Phospho-IRS-1 (Y896) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Y896.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IRS-1 Phospho-Tyr896

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS-1

Background: IRS1, Insulin receptor substrate 1, IRS-1IRS1 encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Molecular Weight: 170kD

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process