ITGA5 antibody (Cleaved-Phe42)

Catalog No. ABIN7221884

The Rabbit Polyclonal anti-ITGA5 antibody is suitable to detect ITGA5 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for ITGA5 antibody (Cleaved-Phe42) (ABIN7221884)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ITGA5 Antibody

Binding Specificity: AA 40-120, Cleaved-Phe42

Purpose: Rabbit Anti-Cleaved-Integrin α5 HC (F42) Polyclonal Antibody

Specificity: Cleaved-Integrin α5 HC (F42) Polyclonal Antibody detects endogenous levels of fragment of activated Integrin α5 HC protein resulting from cleavage adjacent to F42.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Integrin alpha5 HC at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: Integrin alpha5 HC

Background: ITGA5, FNRA, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD antigen CD49eThe product of ITGA5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

Molecular Weight: 92kD

Gene ID: 3678

UniProt: P08648

Pathways: Integrin Complex