ICAM-3/CD50 antibody

Catalog No. ABIN7221636

This Rabbit Polyclonal antibody specifically detects ICAM-3/CD50 in WB, IF and ELISA. It exhibits reactivity toward Human.

Quick Overview for ICAM-3/CD50 antibody (ABIN7221636)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-ICAM-3/CD50 Antibody

Purpose: Rabbit Anti-CD50 Polyclonal Antibody

Specificity: CD50 Polyclonal Antibody detects endogenous levels of CD50 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CD50 around the non-phosphorylation site of S518

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50

Background: ICAM3, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD antigen CD50The intercellular adhesion molecule 3 encoded by ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 42-60kD

Gene ID: 3385

UniProt: P32942