IRF3 antibody (pSer385)

Catalog No. ABIN7221858

The Rabbit Polyclonal anti-IRF3 antibody is suitable to detect IRF3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for IRF3 antibody (pSer385) (ABIN7221858)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-IRF3 Antibody

Binding Specificity: pSer385

Purpose: Rabbit Anti-IRF-3 (phospho Ser385) Polyclonal Antibody

Specificity: Phospho-IRF-3 (S385) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S385.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IRF-3 Phospho-Ser385

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF-3

Background: IRF3, Interferon regulatory factor 3, IRF-3IRF3 encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 48-55kD

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades