IL5RA antibody (N-Term)

Catalog No. ABIN7217964

The Rabbit Polyclonal anti-IL5RA antibody is suitable to detect IL5RA in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for IL5RA antibody (N-Term) (ABIN7217964)

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL5RA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-IL5RA Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-IL-5Rα Polyclonal Antibody

Specificity: IL-5Rα Polyclonal Antibody detects endogenous levels of IL-5Rα protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human IL-5Ralpha

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IL5RA

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name: IL-5Ralpha

Background: IL5RA, IL5R, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, CD125Interleukin-5 receptor subunit alpha encoded by IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.

Molecular Weight: 47kD

Gene ID: 3568

UniProt: Q01344

Pathways: JAK-STAT Signaling