ILF2 antibody

Catalog No. ABIN7229124

This Rabbit Polyclonal antibody specifically detects ILF2 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ILF2 antibody (ABIN7229124)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ILF2 Antibody

Purpose: Rabbit Anti-ILF2 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of ILF2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ILF2 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Alternative Name: ILF2

Background: Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDaThe protein encoded by ILF2 gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.

Molecular Weight: 42kD

Gene ID: 3608

UniProt: Q12905