IRAK1 antibody (pThr100)

Catalog No. ABIN7221842

The Rabbit Polyclonal anti-IRAK1 antibody is suitable to detect IRAK1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for IRAK1 antibody (pThr100) (ABIN7221842)

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRAK1 Antibody

Binding Specificity: pThr100

Purpose: IRAK-1 (phospho Thr100) Polyclonal Antibody

Specificity: Phospho-IRAK-1 (T100) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T100.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human IRAK-1 Phospho-Thr100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK-1

Background: Rabbit Anti-IRAK-1 (phospho Thr100) Polyclonal Antibody,IRAK1, IRAK, Interleukin-1 receptor-associated kinase 1, IRAK-1,IRAK1 encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.,Interleukin-1 receptor-associated kinase 1

Molecular Weight: observerd band 77kDa

Gene ID: 3654

UniProt: P51617

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades