IRAK3 antibody (AA 460-540)

Catalog No. ABIN7219272

The Rabbit Polyclonal anti-IRAK3 antibody is suitable to detect IRAK3 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for IRAK3 antibody (AA 460-540) (ABIN7219272)

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-IRAK3 Antibody

Binding Specificity: AA 460-540

Purpose: Rabbit Anti-IRAK-M Polyclonal Antibody

Specificity: IRAK-M Polyclonal Antibody detects endogenous levels of IRAK-M protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human IRAK-M at AA range: 460-540

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IRAK3

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Alternative Name: IRAK-M

Background: IRAK3, Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, IRAK-MIRAK3 encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. Interleukin-1 receptor-associated kinase 3 is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.

Molecular Weight: 68kD

Gene ID: 11213

UniProt: Q9Y616

Pathways: TLR Signaling, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades