JAK2/3 antibody (pTyr939, pTyr966)

Catalog No. ABIN7229208

The Rabbit Polyclonal anti-JAK2/3 antibody (ABIN7229208) specifically detects JAK2/3 in ELISA, WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for JAK2/3 antibody (pTyr939, pTyr966) (ABIN7229208)

Target: JAK2/3 (Janus Kinase 2/3 (JAK2/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2/3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-JAK2/3 Antibody

Binding Specificity: pTyr939, pTyr966

Purpose: Rabbit Anti-JAK2/3 (Phospho-Tyr966/939) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of JAK2/3 when Phospho occurs at Tyr966/939(human) or Tyr966/935(mouse/rat) , It doesn't react with total protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human JAK2/3 (Phospho-Tyr966/939) Antibody protein at AA range: T966

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for JAK2/3

Target: JAK2/3 (Janus Kinase 2/3 (JAK2/3))

Alternative Name: JAK2/3

Background: Tyrosine-protein kinase JAK2/JAK3, Janus kinase 2/Janus kinase 3, JAK-2/JAK-3JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders. The variant is a known activating mutation, and activated JAK2 is sufficient to drive myeloproliferative disorders in mouse models. V617F, while most recurrent, is not the only mechanism by which JAK2 can be activated in patients. JAK2 is now one of the first diagnostic markers tested upon diagnosis with a myeloproliferative disorder.

Gene ID: 1132, 1124

UniProt: O60674, P52333