KRT8 antibody (pSer73)

Catalog No. ABIN7212932

The Rabbit Polyclonal anti-KRT8 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect KRT8 in samples from Human and Mouse.

Quick Overview for KRT8 antibody (pSer73) (ABIN7212932)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KRT8 Antibody

Binding Specificity: pSer73

Purpose: Cytokeratin 8 (phospho Ser73) Polyclonal Antibody

Specificity: Phospho-Cytokeratin 8 (S73) Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein only when phosphorylated at S73.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cytokeratin 8 Phospho-Ser73

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Rabbit Anti-Cytokeratin 8 (phospho Ser73) Polyclonal Antibody,KRT8, CYK8, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8,KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. Keratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for KRT8.,Keratin type II cytoskeletal 8

Molecular Weight: observerd band 55kDa

Gene ID: 3856

UniProt: P05787