LATS1,2 (pThr1041), (pThr1079) antibody

Catalog No. ABIN7225824

This Rabbit Polyclonal antibody specifically detects LATS1,2 in ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for LATS1,2 (pThr1041), (pThr1079) antibody (ABIN7225824)

Target: LATS1,2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: pThr1041, pThr1079

Purpose: Rabbit Anti-LATS1/2 (phospho Thr1079/1041) Polyclonal Antibody

Specificity: Phospho-LATS1/2 (T1079/1041) Polyclonal Antibody detects endogenous levels of LATS1/2 protein only when phosphorylated at T1079/1041.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human LATS1/2 Phospho-Thr1079/1041

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: LATS1,2

Alternative Name: LATS1/2

Background: LATS1, WARTS, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, LATS2, KPM, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor hoThe protein encoded by LATS1 (large tumor suppressor kinase 1) is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for LATS1.

Gene ID: 9113, 26524

UniProt: O95835, Q9NRM7