LATS1,2 (pThr1041), (pThr1079) antibody

Catalog No. ABIN7229358

The Rabbit Polyclonal anti-LATS1,2 antibody is suitable to detect LATS1,2 in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for LATS1,2 (pThr1041), (pThr1079) antibody (ABIN7229358)

Target: LATS1,2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: pThr1041, pThr1079

Purpose: Rabbit Anti-LATS1/2 (Phospho-Thr1079/1041) Polyclonal Antibody

Specificity: This antibody detects endogenous levels of LATS1/2 when Phospho occurs at Thr1079/1041

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human LATS1/2 (Phospho-Thr1079/1041) Antibody protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: LATS1,2

Alternative Name: LATS1/2

Background: Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-wartsLATS1 (Large Tumor Suppressor Kinase 1) is a Protein Coding gene. Diseases associated with LATS1 include Tetraploidy and Soft Tissue Sarcoma. Among its related pathways are DNA Damage and Mesodermal Commitment Pathway. The protein encoded by LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Molecular Weight: 140kD

Gene ID: 9113

UniProt: O95835