LGMN antibody (AA 60-140)

Catalog No. ABIN7229390

The Rabbit Polyclonal anti-LGMN antibody (ABIN7229390) specifically detects LGMN in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LGMN antibody (AA 60-140) (ABIN7229390)

Target: LGMN (Legumain (LGMN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGMN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LGMN Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-LGMN Polyclonal Antibody

Specificity: The antibody detects endogenous levels of LGMN protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human LGMN protein at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LGMN

Target: LGMN (Legumain (LGMN))

Alternative Name: LGMN

Background: Legumain, Asparaginyl endopeptidase, Protease, cysteine 1LGMN encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of LGMN may be associated with the majority of solid tumor types. LGMN has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Molecular Weight: 47kD

Gene ID: 5641

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades