LIG3 antibody (AA 110-190)

Catalog No. ABIN7214588

The Rabbit Polyclonal anti-LIG3 antibody is suitable to detect LIG3 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for LIG3 antibody (AA 110-190) (ABIN7214588)

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LIG3 Antibody

Binding Specificity: AA 110-190

Purpose: Rabbit Anti-DNA Ligase III Polyclonal Antibody

Specificity: DNA Ligase III Polyclonal Antibody detects endogenous levels of DNA Ligase III protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DNA Ligase III at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: DNA Ligase III

Background: LIG3, DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3LIG3 is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. DNA ligase 3 encoded by LIG3 is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 100kD

Gene ID: 3980

UniProt: P49916

Pathways: DNA Damage Repair