MAD2 antibody (AA 60-140)

Catalog No. ABIN7215660

This Rabbit Polyclonal antibody specifically detects MAD2 in WB, IP and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for MAD2 antibody (AA 60-140) (ABIN7215660)

Target: MAD2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-MAD2 Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-MAD2 Polyclonal Antibody

Specificity: MAD2 Polyclonal Antibody detects endogenous levels of MAD2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MAD2 at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IP 1:200-500,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAD2

Target: MAD2

Background: MAD2L1, MAD2, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

Molecular Weight: 25kD

Gene ID: 4085

UniProt: Q13257