ME3 antibody (AA 520-600)

Catalog No. ABIN7219170

This Rabbit Polyclonal antibody specifically detects ME3 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for ME3 antibody (AA 520-600) (ABIN7219170)

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ME3 Antibody

Binding Specificity: AA 520-600

Purpose: ME3 Polyclonal Antibody

Specificity: ME3 Polyclonal Antibody detects endogenous levels of ME3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human ME3 at AA range: 520-600

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ME3

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Alternative Name: ME3

Background: Rabbit Anti-ME3 Polyclonal Antibody,ME3, NADP-dependent malic enzyme, mitochondrial, NADP-ME, Malic enzyme 3,Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.,NADP-dependent malic enzyme mitochondrial

Molecular Weight: observerd band 67kDa

Gene ID: 10873

UniProt: Q16798

Pathways: Warburg Effect