MKNK1 antibody (pThr385)

Catalog No. ABIN7225548

The Rabbit Polyclonal anti-MKNK1 antibody is suitable to detect MKNK1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for MKNK1 antibody (pThr385) (ABIN7225548)

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKNK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MKNK1 Antibody

Binding Specificity: pThr385

Purpose: Rabbit Anti-Mnk1 (phospho Thr385) Polyclonal Antibody

Specificity: Phospho-Mnk1 (T385) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T385.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Mnk1 Phospho-Thr385

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MKNK1

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Alternative Name: Mnk1

Background: MKNK1, MNK1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, MAPK signal-integrating kinase 1, Mnk1MKNK1 (MAP kinase interacting serine/threonine kinase 1) encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for MKNK1.

Molecular Weight: 42kD

Gene ID: 8569

UniProt: Q9BUB5

Pathways: MAPK Signaling, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor