MKNK1 antibody (pThr250)

Catalog No. ABIN7225550

The Rabbit Polyclonal anti-MKNK1 antibody (ABIN7225550) specifically detects MKNK1 in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MKNK1 antibody (pThr250) (ABIN7225550)

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKNK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MKNK1 Antibody

Binding Specificity: pThr250

Purpose: Rabbit Anti-Mnk1 (phospho Thr250) Polyclonal Antibody

Specificity: Phospho-Mnk1 (T250) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T250.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Mnk1 Phospho-Thr250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MKNK1

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Alternative Name: Mnk1

Background: MKNK1, MNK1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, MAPK signal-integrating kinase 1, Mnk1MKNK1 (MAP kinase interacting serine/threonine kinase 1) encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for MKNK1.

Gene ID: 8569

UniProt: Q9BUB5

Pathways: MAPK Signaling, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor