MDM4-binding Protein antibody (pSer367)

Catalog No. ABIN7222412

This Rabbit Polyclonal antibody specifically detects MDM4-binding Protein in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MDM4-binding Protein antibody (pSer367) (ABIN7222412)

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM4-binding Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MDM4-binding Protein Antibody

Binding Specificity: pSer367

Purpose: MDMX (phospho Ser367) Polyclonal Antibody

Specificity: Phospho-MDMX (S367) Polyclonal Antibody detects endogenous levels of MDMX protein only when phosphorylated at S367.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MDMX Phospho-Ser367

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Alternative Name: MDMX

Background: Rabbit Anti-MDMX (phospho Ser367) Polyclonal Antibody,MDM4, MDMX, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4,MDM4 encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.,Protein Mdm4

Molecular Weight: observerd band 80kDa

Gene ID: 4194

UniProt: O15151

Pathways: Cell Division Cycle