MED17 antibody (AA 110-190)

Catalog No. ABIN7214348

This anti-MED17 antibody is a Rabbit Polyclonal antibody detecting MED17 in WB, ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for MED17 antibody (AA 110-190) (ABIN7214348)

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED17 Antibody

Binding Specificity: AA 110-190

Purpose: CRSP77 Polyclonal Antibody

Specificity: CRSP77 Polyclonal Antibody detects endogenous levels of CRSP77 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human CRSP77 at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MED17

Target: MED17 (Mediator Complex Subunit 17 (MED17))

Alternative Name: CRSP77

Background: Rabbit Anti-CRSP77 Polyclonal Antibody,MED17, ARC77, CRSP6, DRIP77, DRIP80, TRAP80, Mediator of RNA polymerase II transcription subunit 17, Activator-recruited cofactor 77 kDa component, ARC77, Cofactor required for Sp1 transcriptional activation subunit 6, CRSP complex subunit,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator complex subunit 17 encoded by MED17 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.,Mediator of RNA polymerase II transcription subunit 17

Molecular Weight: observerd band 73kDa

Gene ID: 9440

UniProt: Q9NVC6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha