Meprin B antibody

Catalog No. ABIN7229644

The Rabbit Polyclonal anti-Meprin B antibody (ABIN7229644) specifically detects Meprin B in WB and ELISA. The antibody is reactive with Rat, Human and Mouse samples.

Quick Overview for Meprin B antibody (ABIN7229644)

Target: Meprin B (MEP1B) (Meprin A, beta (MEP1B))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Meprin B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Meprin B Antibody

Purpose: Rabbit Anti-MEP1B Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MEP1B protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MEP1B protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Meprin B

Target: Meprin B (MEP1B) (Meprin A, beta (MEP1B))

Alternative Name: MEP1B

Background: Meprin A subunit beta, Endopeptidase-2, Meprin B, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta, PABA peptide hydrolase, PPH betaMeprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.

Molecular Weight: 77kD

Gene ID: 4225

UniProt: Q16820