Meprin B antibody (AA 73-489)

Catalog No. ABIN8009154

The Rabbit Polyclonal anti-Meprin B antibody is suitable to detect Meprin B in samples from Rat, Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Meprin B antibody (AA 73-489) (ABIN8009154)

Target: Meprin B (MEP1B) (Meprin A, beta (MEP1B))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Meprin B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Meprin B Antibody

Binding Specificity: AA 73-489

Purpose: Anti-MEP1B Antibody

Predicted Reactivity: Human MEP1B shares 82.3%,81.5% amino acid (aa) sequence identity with mouse,rat MEP1B,respectively.

Characteristics: Anti-MEP1B Antibody. Tested in WB, IF, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MEP1B recombinant protein (Position: H73-Q489). Human MEP1B shares 82.3% and 81.5% amino acid (aa) sequence identity with mouse and rat MEP1B, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunofluorescence, 5 μg/mL, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Meprin B

Target: Meprin B (MEP1B) (Meprin A, beta (MEP1B))

Alternative Name: MEP1B

Background:

Background: Meprin A subunit beta is a protein that in humans is encoded by the MEP1B gene. Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.

Gene Full Name: meprin A subunit beta

Molecular Weight: 97 kDa

Gene ID: 4225

UniProt: Q16820