MANF antibody (Internal Region)

Catalog No. ABIN7218154

This Rabbit Polyclonal antibody specifically detects MANF in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MANF antibody (Internal Region) (ABIN7218154)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MANF Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-ARP Polyclonal Antibody

Specificity: ARP Polyclonal Antibody detects endogenous levels of ARP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human ARP

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: ARP

Background: MANF, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMETMesencephalic astrocyte-derived neurotrophic factor encoded by MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors, however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.

Molecular Weight: 20kD

Gene ID: 7873

UniProt: P55145