Manic Fringe antibody (AA 30-110)

Catalog No. ABIN7222460

The Rabbit Polyclonal anti-Manic Fringe antibody has been validated for WB and ELISA. It is suitable to detect Manic Fringe in samples from Human, Mouse and Rat.

Quick Overview for Manic Fringe antibody (AA 30-110) (ABIN7222460)

Target: Manic Fringe (MFNG) (MFNG)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Manic Fringe antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Manic Fringe Antibody

Binding Specificity: AA 30-110

Purpose: Manic Fringe Polyclonal Antibody

Specificity: Manic Fringe Polyclonal Antibody detects endogenous levels of Manic Fringe protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Manic Fringe at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Manic Fringe

Target: Manic Fringe (MFNG) (MFNG)

Alternative Name: Manic Fringe

Background: Rabbit Anti-Manic Fringe Polyclonal Antibody,MFNG, Beta-1, 3-N-acetylglucosaminyltransferase manic fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase,MFNG is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling.,Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Molecular Weight: observerd band 38kDa

Gene ID: 4242

UniProt: O00587

Pathways: Notch Signaling