MITF antibody (pSer180)

Catalog No. ABIN7222476

The Rabbit Polyclonal anti-MITF antibody is suitable to detect MITF in samples from Human, Mouse and Monkey. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for MITF antibody (pSer180) (ABIN7222476)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-MITF Antibody

Binding Specificity: pSer180

Purpose: Rabbit Anti-MITF (phospho Ser180) Polyclonal Antibody

Specificity: Phospho-MITF (S180) Polyclonal Antibody detects endogenous levels of MITF protein only when phosphorylated at S180.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MITF Phospho-Ser180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32MITF encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 52kD

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding