MAP4 antibody

Catalog No. ABIN7222328

The Rabbit Polyclonal anti-MAP4 antibody is suitable to detect MAP4 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for MAP4 antibody (ABIN7222328)

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MAP4 Antibody

Purpose: Rabbit Anti-MAP-4 Polyclonal Antibody

Specificity: MAP-4 Polyclonal Antibody detects endogenous levels of MAP-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MAP-4 around the non-phosphorylation site of S696

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAP4

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Alternative Name: MAP-4

Background: MAP4, Microtubule-associated protein 4, MAP-4The protein encoded by MAP4 is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 121kD

Gene ID: 4134

UniProt: P27816

Pathways: p53 Signaling, Microtubule Dynamics