MRPL47 antibody (AA 160-240)

Catalog No. ABIN7223706

This Rabbit Polyclonal antibody specifically detects MRPL47 in WB and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for MRPL47 antibody (AA 160-240) (ABIN7223706)

Target: MRPL47 (Mitochondrial Ribosomal Protein L47 (MRPL47))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL47 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MRPL47 Antibody

Binding Specificity: AA 160-240

Purpose: Rabbit Anti-MRP-L47 Polyclonal Antibody

Specificity: MRP-L47 Polyclonal Antibody detects endogenous levels of MRP-L47 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human MRP-L47 at AA range: 160-240

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPL47

Target: MRPL47 (Mitochondrial Ribosomal Protein L47 (MRPL47))

Alternative Name: MRP-L47

Background: MRPL47, NCM1, CGI-204, 39S ribosomal protein L47, mitochondrial, L47mt, MRP-L47, Nasopharyngeal carcinoma metastasis-related protein 1Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL47 (mitochondrial ribosomal protein L47) encodes a 39S subunit protein. MRPL47 is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.

Molecular Weight: 30kD

Gene ID: 57129

UniProt: Q9HD33