MRPS18A antibody (AA 50-130)

Catalog No. ABIN7223394

The Rabbit Polyclonal anti-MRPS18A antibody is suitable to detect MRPS18A in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for MRPS18A antibody (AA 50-130) (ABIN7223394)

Target: MRPS18A (Mitochondrial Ribosomal Protein S18A (MRPS18A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS18A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MRPS18A Antibody

Binding Specificity: AA 50-130

Purpose: Rabbit Anti-MRP-S18A Polyclonal Antibody

Specificity: MRP-S18A Polyclonal Antibody detects endogenous levels of MRP-S18A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MRP-S18A at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPS18A

Target: MRPS18A (Mitochondrial Ribosomal Protein S18A (MRPS18A))

Alternative Name: MRP-S18A

Background: MRPS18A, 28S ribosomal protein S18a, mitochondrial, MRP-S18-a, Mrps18a, S18mt-a, 28S ribosomal protein S18-3, mitochondrial, MRP-S18-3Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS18A (mitochondrial ribosomal protein S18A) encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to MRPS18A is found on chromosome 3p. Alternative splicing results in multiple transcript variants.

Molecular Weight: 27-30kD

Gene ID: 55168

UniProt: Q9NVS2