MAPK6 antibody (pSer189)

Catalog No. ABIN7223596

The Rabbit Polyclonal anti-MAPK6 antibody is suitable to detect MAPK6 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for MAPK6 antibody (pSer189) (ABIN7223596)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MAPK6 Antibody

Binding Specificity: pSer189

Purpose: Rabbit Anti-ERK 3 (phospho Ser189) Polyclonal Antibody

Specificity: Phospho-ERK 3 (S189) Polyclonal Antibody detects endogenous levels of ERK 3 protein only when phosphorylated at S189.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ERK 3 Phospho-Ser189

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: ERK 3

Background: MAPK6, ERK3, PRKM6, Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPKThe protein encoded by MAPK6 (mitogen-activated protein kinase 6)is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Molecular Weight: 90kD

Gene ID: 5597

UniProt: Q16659

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C