MYF5 antibody (pSer49)

Catalog No. ABIN7222604

This Rabbit Polyclonal antibody specifically detects MYF5 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for MYF5 antibody (pSer49) (ABIN7222604)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MYF5 Antibody

Binding Specificity: pSer49

Purpose: Myf-5 (phospho Ser49) Polyclonal Antibody

Specificity: Phospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Myf-5 Phospho-Ser49

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: Myf-5

Background: Rabbit Anti-Myf-5 (phospho Ser49) Polyclonal Antibody,MYF5, BHLHC2, Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2,MYF5 (Myogenic Factor 5) is a Protein Coding gene. Diseases associated with MYF5 include skeletal muscle cancer and botryoid rhabdomyosarcoma. Among its related pathways are Signaling pathways regulating pluripotency of stem cells and Mesenchymal Stem Cell Differentiation Pathways and Lineage-specific Markers. GO annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1.,Myogenic factor 5

Molecular Weight: observerd band 38kDa

Gene ID: 4617

UniProt: P13349

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development