MYLK antibody (pTyr464)

Catalog No. ABIN7222614

This Rabbit Polyclonal antibody specifically detects MYLK in ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MYLK antibody (pTyr464) (ABIN7222614)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYLK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MYLK Antibody

Binding Specificity: pTyr464

Purpose: Rabbit Anti-MYLK (phospho Tyr464) Polyclonal Antibody

Specificity: Phospho-MYLK (Y464) Polyclonal Antibody detects endogenous levels of MYLK protein only when phosphorylated at Y464.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MYLK Phospho-Tyr464

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: MYLK

Background: MYLK, MLCK, MLCK1, MYLK1, Myosin light chain kinase, smooth muscle, MLCK, s mMLCK, Kinase-related protein, KRP, TelokinMYLK, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. MYLK encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

Gene ID: 4638

UniProt: Q15746