NEK7 antibody (AA 70-150)

Catalog No. ABIN7219686

The Rabbit Polyclonal anti-NEK7 antibody is suitable to detect NEK7 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for NEK7 antibody (AA 70-150) (ABIN7219686)

Target: NEK7

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NEK7 Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-Nek7 Polyclonal Antibody

Specificity: Nek7 Polyclonal Antibody detects endogenous levels of Nek7 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Nek7 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEK7

Target: NEK7

Alternative Name: Nek7

Background: NEK7, Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.

Molecular Weight: 35kD

Gene ID: 140609

UniProt: Q8TDX7

Pathways: Inflammasome