NEURL antibody (pTyr877)

Catalog No. ABIN7213052

The Rabbit Polyclonal anti-NEURL antibody (ABIN7213052) specifically detects NEURL in WB, ELISA, IHC, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NEURL antibody (pTyr877) (ABIN7213052)

Target: NEURL (Neuralized Homolog (Drosophila) (NEURL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEURL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-NEURL Antibody

Binding Specificity: pTyr877

Purpose: Rabbit Anti-Neu (phospho Tyr877) Polyclonal Antibody

Specificity: Phospho-Neu (Y877) Polyclonal Antibody detects endogenous levels of Neu protein only when phosphorylated at Y877.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Neu Phospho-Tyr877

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEURL

Target: NEURL (Neuralized Homolog (Drosophila) (NEURL))

Alternative Name: Neu

Background: ERBB2, HER2, MLN19, NEU, NGL, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD antigen CD340ERBB2 encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Erb-b2 receptor tyrosine kinase 2 has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of ERBB2 has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Molecular Weight: 180kD

Gene ID: 2064

UniProt: P04626

Pathways: Notch Signaling, EGFR Signaling Pathway