NEUROD1 antibody (pSer274)

Catalog No. ABIN7222704

This Rabbit Polyclonal antibody specifically detects NEUROD1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NEUROD1 antibody (pSer274) (ABIN7222704)

Target: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEUROD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEUROD1 Antibody

Binding Specificity: pSer274

Purpose: Neuro D (phospho Ser274) Polyclonal Antibody

Specificity: Phospho-Neuro D (S274) Polyclonal Antibody detects endogenous levels of Neuro D protein only when phosphorylated at S274.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Neuro D Phospho-Ser274

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NEUROD1

Target: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Alternative Name: Neuro D

Background: Rabbit Anti-Neuro D (phospho Ser274) Polyclonal Antibody,NEUROD1, BHLHA3, NEUROD, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3,NEUROD1 encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.,Neurogenic differentiation factor 1

Molecular Weight: observerd band 36kDa

Gene ID: 4760

UniProt: Q13562

Pathways: Dopaminergic Neurogenesis, Hormone Transport, Carbohydrate Homeostasis