Nibrin antibody

Catalog No. ABIN7222658

This Rabbit Polyclonal antibody specifically detects Nibrin in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Nibrin antibody (ABIN7222658)

Target: Nibrin (NBN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nibrin Antibody

Purpose: Nibrin Polyclonal Antibody

Specificity: Nibrin Polyclonal Antibody detects endogenous levels of Nibrin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Nibrin around the non-phosphorylation site of S278

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: Nibrin

Background: Rabbit Anti-Nibrin Polyclonal Antibody,NBN, NBS, NBS1, P95, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1,Mutations in NBN are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.,Nibrin

Molecular Weight: observerd band 85kDa

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response