NUMB antibody (Internal Region)

Catalog No. ABIN7218174

The Rabbit Polyclonal anti-NUMB antibody is suitable to detect NUMB in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for NUMB antibody (Internal Region) (ABIN7218174)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NUMB Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-NUMB Polyclonal Antibody

Specificity: NUMB Polyclonal Antibody detects endogenous levels of NUMB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human NUMB

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IF/ICC1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background: NUMB, Protein numb homolog, h-Numb, Protein S171Protein numb homolog encoded by NUMB plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.

Molecular Weight: 70kD

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization