ITK antibody (pTyr512)

Catalog No. ABIN7221916

The Rabbit Polyclonal anti-ITK antibody is suitable to detect ITK in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ITK antibody (pTyr512) (ABIN7221916)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ITK Antibody

Binding Specificity: pTyr512

Purpose: Rabbit Anti-Emt (phospho Tyr512) Polyclonal Antibody

Specificity: Phospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Emt Phospho-Tyr512

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: Emt

Background: ITK, EMT, LYK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase LykITK encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Molecular Weight: 72kD

Gene ID: 3702

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway