ORC2 antibody (AA 180-260)

Catalog No. ABIN7230402

The Rabbit Polyclonal anti-ORC2 antibody (ABIN7230402) specifically detects ORC2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ORC2 antibody (AA 180-260) (ABIN7230402)

Target: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ORC2 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-ORC2 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of ORC2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ORC2 protein at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ORC2

Target: ORC2 (Origin Recognition Complex Subunit 2 (ORC2))

Alternative Name: ORC2

Background: Origin recognition complex subunit 2ORC is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by ORC2 is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.

Molecular Weight: 63kD

Gene ID: 4999

UniProt: Q13416